ࡱ> z  !"#$%&'()*+,-./013456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYb[\]^_`acdefghijklmnopqrstuvwxy{|}~Root Entry F sSummaryInformation(hDocumentSummaryInformation8 WordDocument2   Oh+'08(4L d t  0FOR RESEARCH USE ONLY. 0FE076201A4D470Normal Nwmyuir152@ԫ@s@4SPM l$<WPS Office_11.1.0.9305_F1E327BC-269C-435d-A152-05C5408002CA ՜.+,D՜.+,\  N* (\dlKSOProductBuildVer2052-11.1.0.93050TableZ$Data "UWpsCustomData DdI6#f  s 4A(8? VGr 2"bo?:B-N1Ҙno?:B-N1ҘPNG  IHDR'jqsBITO pHYs++IDATxy|egIӋn)wSYJ]E]eEDmlYz-mu=v貲 6KԔWhh+(gz^)m8$$d4<>P""U T"6Pu@A bU T"6Pu@A bU T"62/] W'&MNN49%q|`E@mݳDDq\_4h\bBFJ䔄 B!TkRp~8"Ap) 12!s̑qFƆˡ!CP]Lں{\AlT㿈"M1*6sT 5n,;'' n;ʕc ud\D\\_]=׽۾7EUNvZjrLٺ>`޺pڨW&B]]XѮN'GoNI_8gb#r{U`s-Ayʔ>8e al?r\94$X&K!A!L" e`i9}͞kfAr(YMGqxLӷU&[ }{l ʥ)53܂cs$KKN}re[#="`Yh,4X, i ȭ)F,PM\4eBj}FUՙk'=y;.cO|w~_K&/h넵n}t M]^n{&I9.7)c"仟 @n䠧hUgI~1eNIp:rU_pV]Z|½Je,׻]=Xo=}~!ANO)uo|dw"2&6 '{4׳ԡ] G/68(Ka fN7S5>q翪o֪:(5 5V__=Oװ{/\bl86y\lC5]r dL_9}B"(#WZ6X=ssO]=w<@ l`;7iݮIY2WMU4.~Zwhg& .Y0gd$R|p_^UU7#w|,@ lbFXﯟذr];3'Kac񣖓.LwEރW[M':}܌Y0u?ˆzfE/^VtE={YTn8qփ֪A:}Lg~AĀullb;"2qIƲ%Ɔ lyCcUF[MAlIKc"D$89hS_JX>-e8]jZ}0".2dxafKC llj;7h8bܟbmDaƃvncOhs|@3<>YhP=.|p\-3JJ, g0 3x ?"B&b0wE A`1EҮ\7D~PAgC S~#Jqc aY6jd0Z+CWF媋e6AWcIfT]~~>lyy^'$j["ǜ(3>OAsנy>R݄a i@ 1꿟7L:SYY骱DA2#gz>`6y1#'f9@VR!D K_7_NV9`f:H+͌0ц 8+rJk\NJB4pFS1)aANbnjLaaaVV :6ZraxD4_GnRd28h3)xl[q)lLa[29ScQ#RjT*b~OC,%M zz8#:GP-ɛ}Z1)--Ϊ~L$f`TӲ l™>Ƿ_mAu0(Y/ZLj!Qj v;(O [dzኦU'T4ifb*GY_b<_<wegaW4ުr낅haLJz,tױNU9 (˝agJ;܏qiauGFl, 0S6d2[da-T+uTnosxI P.(..ob(NGѨRȃ:@'ߥ8:>n }e׎RN zqMyy97LjjjrrrȋSɾ0Fr0qolu‚rB9[#f4\^kZKRTM6UEo)+/J d2'3B'TGS8nGs4%X&}z\ߣnSd1Pv˕ĎeI@TP'qRd9Rȡ]?6DH]`N#(n~Yx_oDii)xܖ؁:>V9"ZVP.aF(!SBAIR?u4ߚmKTgpoC1:4M S$} uZB$R -FJfو[o F\yl=qᖜQkI軫g/6[.H{3~rkEGGCAn'O LOAQ$u@E9}}·7 QxG;ǛKAnmGLS]0IKs\:j2:|- ^v"l/]_k6Xmv8 ,|IǛ[e+gF9ĉ`GBQVVFlMO/$"FS[Cv`􄨾yr3oR*ίzxZ* Q6kM&]~A:pb+ >i,_4E;O|5kKd*b~_(CVQQ!e웜Aj*3B;%KV]lγ_'L 3>Ա4uJ~rU < Z5p.J3%ƽwU6rߕ}jbɀ.x JM/~1붮86w֣ gFςDՙ%W>NWXXXVVFu`&n+ p$9ش… ij 0<@MT5v4"$wMM_0Y6WYuzDUm ? 6\1j8:[]ݻhJe0fsMMR$":»_Wu7;'>r_NK[3.3HTn 0fNэ? K舝m/^R{ 7u?x~yLOZʇG6_:=Bi> OPee%c|ǒj'8Ku4uZŏ'_Y4k#/8x,ڶ7_>p擋;L- "X,P({u^\\c`檝I7Ŕ%N r7_Gt|rt%u쎢yM9*Yn-1ɱiɱcS$Ǧ%ņ= S# TGPPiWj YO7rM}5$NOO'i 2 ',M7V|u>w}+#PM76%.-9|%s_D.^w5z,\hً^Y8km}N_!_āؔ1qiI6&pUhj}g[Tuݏ߼zt8ѝC+@WgFMs&j~{%GNYqtGcuͼ'PPRA@_ C!:dH;H@A bU]Wwd^IENDB`9Dd   vA0(8 OV)T60RE8O)DNYG2(Z~(GJKVGr 13"R8,iN:+ʼd Mw8Fo8,iN:+ʼd MJFIF``C     C   " }!1AQa"q2#BR$3br %&'()*456789:CDEFGHIJSTUVWXYZcdefghijstuvwxyz w!1AQaq"2B #3Rbr $4%&'()*56789:CDEFGHIJSTUVWXYZcdefghijstuvwxyz ?S((7 Z)7(4RI{T);{Q/hR3@ E&x-QM.?(9MQM=*7.@ E4FP4QEQEQEQEQEQEQEQEQEQEQEQEQEQL3"R2x?3AU|eGM?v=ncV<;L?_du#(tQUU $9 EYj{F~1x?3ksbj8)?wP8, > 3 EW⌊sW|?Xտ|>žfsW?ciW,T}?(g5r|?¢ O.E_@I^P]?:Qci/ߌ_Xъk۰~0xXq  Q^P?jb~=#1;@EyRh㴿x {;@Ey_5w/,^ž ]K?WW|)x {;@Ey_5w/,^ž ]K?WW|)x {;@Ey_5w/,^ž ]K?WW|)x {;@Ey_5w/,^ž ]K?W7aIOƽ+Jl.Ro 4RU)`I  "-EQEQEQEQEC<mKi6Vڽo:Ybht w# <׏C~>nCam/u젍&~$װItڄ 1y[>k:_>x^PF4'x/Z8HmS>Ux !05 mqe$m(%,*\XJD@3jo 7emĚ6ףhӵKDn NUGG{Yy7s  \A IJ.E}]@U C%??^W@U C%??^W@U C%??^W@U C%??^W@U C%??^W@U C%??^W@U C%??^W@U C%??^W@U C%??^W@U C%??^W@U C%??^W@U C%??^W@U C%??^W@U C%??^W@U C%??^W@U C%??^W@U C%??^W@U C%??^W@U C%??^W@0~?7~M+O4Nzͭ퟇,dHcYXA,+ (iߵO5!MX7bf0QEQEQEQEQE_2 <'z-ȵh||ro6>py'|K߭`ҥۿ2ݨf7$~9jkڟm{Pn(Ki)"$U} ٿ[ާ똴F6,uZCwBPI2@ѳyp>yQ?;;n$?7*Rn ]ר\\WմQEQEQEQEQEQEQEQEQEQEQEQEQEdŚ+oĚΟo5R-|BYFO%hZtqmS:LWzum*kHHP;syŭWWX=<MAsc}H6iPJڸ L2\墊+;((((*>2ؙ ԟo4o!Xbf3R~ɿkLѿj( ( ( ( (D /i[faERվ'Ik~]SS(|mgU#_n.Qad_þb_}?OpE_VWGگ[|$/NJqx^ZK y?u6ۻfYz~?s(+_`_ G0/\ohh.Eq?>U?\W7Q W@s(+_`_ G0/\ohh.Eq?>U?\W7Q W@s(+_`_ G0/\ohh.Eq?>U?\O>~7~7xoEpNk,criOJ򏆿o*67/X6xė IoDz+8QC+!!U ڦ2π?qO;2mVԍ=aPpKM U앪x]WZ{z,>˱`~,^misq1P81+JL_%ܽ☬yH ];e' 8ſ>?.{/0?E}SE| W@s(?U0/\oh7PTWʿq?`_ @UQ_*\?h.E}UE| W@s(?U0/\oh7P?k?LjO7Mc7_6~П7"~%7?پ?w^]O7ˑ$f?e}?Mה4QEQEQEQEQEq?Ꮜ4o cF^Zi-ԐE/vV,ېJ>xi<+owO֋-l! 8EO2VU4Yh,4xY?[m3Bml9(8?@QEQEQEQEQEQEQEQEyWc&gHf> ؙ 4O5!MX7bf0QEQEQEQEQE|GO]xJSLZe̚MDا\dּ-i<7K+h-qhq* l؀͕< /I<_{}ƯuhZuv-1*pHʤSúu֥i!ѵuX,tfEa ĵY_(6B_q2t~ÃK?}__(G3G>.g? ((((((((O?g+LUjZi:]Z\ %mX2\|i-γPԼTk-COE\"^y жS̾@%>>ěg2oO𶛳 d*rA,1'KWeEÿ/?mn7M>hd+HKc@J,΁ʱiL]'O{xYmkFJ5z&IDſ>%.;o&7lŤ̚*_:5Z?.Mll<Ծ%><.5ׄѫ;dwF%~~h+!||;~,<]C^S>7~%Q`ۊ–VBHaVcV`ѿ%in>Uѿ%inhw2V(_hw2Vѿ%in>Uѿ%inhw2V(_hw2Vѿ%in>Uѿ%inhw2V(_hw2Vѿ%in>Uѿ%inhw2V(_hw2Vѿ%in>Uѿ%inhw2V(_hw2Vѿ%in>~;~:/vuN _sE%4ؼM}'%?e7GrXɬ|d3Y?d5&hC zQ@Q@Q@Q@Q@>/FcPk9?UifV6>}Bo>T?2k࿆zޅfo2ؙ ԟo4o!Xbf3R~ɿkLѿj( ( ( ( (0_mo{^F$eE (3,gx;>vU;A&$> ~eGN51U/3m " y1[:ٯ F{$MH-n{|LmpV+}o\5 O="niQi/KiJ;yHiX^:?h۫Hu~Hɯ_G⏅ տ׌|ccxkL>*],/2)^D&z?E7(#PTWʿًoP5OF1MU=b S_S5}UE|GTj?j@UQ_*f/)@?OW>5_2[~sg,1ƩUf_1Dl)fm [&EGTKkxw0E#eQ`:h?hg#N|1Oş(N6\ ;MeIf&m4FÀOZW;3.g~t%w Þm9]#x ؛(hը((((>8E|LJ-_yH۾mQEipP2 OPd/0D:>q>K%lmHCqicRGrY0 @ū]6߈gEɧ\j;\^%*m23Ƒ<2k?I-bIiķlc!!i&X6wK/xv~/3U$sRKmsmNh,HR7y\J+"1LѼp>xcyaj[_ xZjfimsdmF*NHx6?8rs0nw<.* (˟$xw&E5Kq9_]l@/aّ$w.õNJYIpsj~|N>/AeT> ˍZI Ӏq_$}P~:·{o Y]yom$hL3Ē?og^jC}x_9ֽm64dyE(}2U G"+pBhget)Rs_tee^1<&zm&$R8gerʹ&Cap8@:B((((((((((((((((*>2ؙ ԟo4o!Xbf3R~ɿkLѿj( ( ( ( ( *74$+B]oOƛy&|l.Rqiu<%(Nr 28jh ?e=kZΟևşhu;v \(<twd<]Ǟ#wm Hw)۸3nt|$G lw2emoL۶mؙی6OsZ$Z;|Qch!E?v?3eƨO%Ds?L:!E?vkY>Qci? 6_jd_G<͗!E?v~H cix /5U> ,Gx\b͹=x΀8߄nx^ 5e{_Ae[~Mn)n,UoSऀŞ!\m!1gMNT|y'.I cC'*,??|?¢'9&lx /5@ cC'*,??|?¢'9&lx /5@ cC'*,??|?¢'9&lx /5@ cC'*,??|?¢'9&lD߲}wd 1eU9Wx3]o<-} h/N4`i%2Ur~O;Oޟ$~cjJV@cIޤW Qۓ͐ϩUBT[X ;G5,(e|?,_e_G<͗bX ;G5,㴟x /5G2/#fPX ;G5,㴟x /5G2/#fPX ;G5,㴟x /5U5Oٛ&au}(uceiOqss5݌xUIhv|cz,~5R%-~͡/?s#~ߵb è>(ȵUeo\žbO}}kȞ)O *>'=4Sm?qwfgS cC'*,??|?¢WQ"#Tx /5HbX ;G5,㴟x /5G2/#fPX ;G5,㴟x /5G2/#fPX ;G5,㴟x /5LoWzgwl @sM~?A7|WԵ; jV#k^UHEfbIJ-S\=3zCᾢW/gv,>Am:`<2 GM]WmKk7Úixt.͌vom`N.ԍFppiO2tSUՀ EQ@Q@Q@Q@--v@d;f?'"Ҏtw^)~.:ضq~g1-cDO(((+95]rD~|%M?+un]ёlzqǚ.[8)y_~Ogwg>w+^Es ~qo 3ѳWA ZOMT4ptXomTX/.YdxGq7d$gsY0CpJOD K袊FEPEP^=ZjԬ[[, /qg,+kx'f ?jwUb%c>1/OjZs3Icҧ xN c SiZT/.a6a%,6aZ:M]6f+7-s<:ʦ#ޝVF|6,Q'ڞ:Ph &EC ( ;7^9'B>$Դɭf˙QLRl=˖WeNO2\ɣi۴1qjY2M12&ܟ7)ϥ>e[EҮtK˟:՚K Nbm#hDW"h[rE۝>.Ӛ+(E.B#8QLaEPUn-̎@8ztǯV(XnfƷQ޷,vx]?춖Q ɹ`bYi@&1\@7C$"t" ] `  Rabbit IL-6 ELISA Kit For the quantitative in vitro determination of Rabbit Interleukin 6 concentrations in serum - plasma - tissue homogenates - other biological fluids FOR LABORATORY RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. This package insert must be read in its entirety before using this product. ELISA ENZYME LINKED IMMUNOSORBENT ASSAY INTENDED USE AND TEST PRINCIPLE This IL-6 ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of IL-6 in the sample, this IL-6 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus IL-6 concentration. The concentration of IL-6 in the samples is then determined by comparing the O.D. of the samples to the standard curve. SAMPLE COLLECTION AND STORAGES Serum - Use a serum separator tube and allow samples to clot for two hours at room temperature or overnight at 4! before centrifugation for 20 minutes at approximately 1000g. Assay freshly prepared serum immediately or store samples in aliquot at -20! or -80! for later use. Avoid repeated freeze/thaw cycles. Plasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000g at 2-8! within 30 minutes of collection. Remove plasma and assay immediately or store samples in aliquot at -20! or -80! for later use. Avoid repeated freeze/thaw cycles. Tissue homogenates - For general information, hemolysis blood may affect the result, so you should rinse the tissues with ice-cold PBS (0.01M, pH=7.4) to remove excess blood thoroughly. Tissue pieces should be weighed and then minced to small pieces which will be homogenized in PBS (the volume depends on the weight of the tissue. 9mL PBS would be appropriate to 1 gram tissue pieces. Some protease inhibitor is recommended to add into the PBS.) with a glass homogenizer on ice. To further break the cells, you can sonicate the suspension with an ultrasonic cell disrupter or subject it to freeze-thaw cycles. The homogenates are then centrifugated for 5minutes at 5000g to get the supernate. Cell culture supernates and other biological fluids - Centrifuge samples for 20 minutes at 1000g. Remove particulates and assay immediately or store samples in aliquot at -20! or -80! for later use. Avoid repeated freeze/thaw cycles. Note: The samples shoule be centrifugated dequately and no hemolysis or granule was allowed. MATERIALS REQUIRED BUT NOT SUPPLIED 1. 37 ! incubator 2. Standard microplate reader capable of measuring absorbance at 450 nm 3. Precision pipettes, disposable pipette tips and Absorbent paper 4. Distilled or deionized water REAGENTS PROVIDED All reagents provided are stored at 2-8C. Refer to the expiration date on the label. Name96 determinations48 determinationsMICROTITER PLATE8*12strips8*6stripsSTANDARD6 vial 0.3ml/vial0.3ml/vialSAMPLE DILUENT6.0ml3.0mlENZYME CONJUGATE10.0ml5.0mlWASH SOLUTION25ml15mlSUBSTRATE A6.0ml3.0mlSUBSTRATE B6.0ml3.0mlSTOP SOLUTION6.0ml3.0mlClosure plate membrane22User manual11Sealed bags11Note: 1. Standard concentration was followed by: 120, 60, 30, 15, 7.5, 3.75 pg/mL. 2. Ifsamplesgeneratevalueshigherthanthehigheststandard, pleasedilutethe sampleswithSampleDiluentandrepeattheassay. PRECAUTIONS Do not substitute reagents from one kit lot to another. Standard, conjugate and microtiter plates are matched for optimal performance. Use only the reagents supplied by manufacturer. Allow kit reagents and materials to reach room temperature (20-25C) before use. Do not use water baths to thaw samples or reagents. Do not use kit components beyond their expiration date. Use only deionized or distilled water to dilute reagents. Do not remove microtiter plate from the storage bag until needed. Unused strips should be stored at 2-8C in their pouch with the desiccant provided. Use fresh disposable pipette tips for each transfer to avoid contamination. Do not mix acid and sodium hypochlorite solutions. Serum and plasma should be handled as potentially hazardous and capable of transmitting disease. Disposable gloves must be worn during the assay procedure, since no known test method can offer complete assurance that products derived from Rat blood will not transmit infectious agents. Therefore, all blood derivatives should be considered potentially infectious and good laboratory practices should be followed. All samples should be disposed of in a manner that will inactivate viruses. Liquid Waste: Add sodium hypochlorite to a final concentration of 1.0%. The waste should be allowed to stand for a minimum of 30 minutes to inactivate the viruses before disposal. Substrate Solution is easily contaminated. If bluish prior to use, do not use. Substrate B contain 20% acetone, keep this reagent away from sources of heat or flame. Remove all kit reagents from refrigerator and allow them to reach room temperature ( 20-25C). REAGENT PREPARATION AND STORAGE Wash Solution (1X) - Dilute 1 volume of Wash solution (20X) with 19 volumes of deionized or distilled water. Wash Solution is stable for 1 month at 2-8C. ASSAY PROCEDURE 1. Prepare all reagents before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microtiter plate. 2. Add 50l of Standard or Sample to the appropriate wells. Blank well doesn t add anyting. 3. Add 100l of Enzymeconjugate to standard wells and sample wells except the blank well, cover with an adhesive strip and incubate for 60 minutes at 37C. 4. Wash the Microtiter Plate 4 times. Manual Washing - Remove incubation mixture by aspirating contents of the plate into a sink or proper waste container. Using a squirt bottle, fill each well completely with Wash Solution (1X), then aspirate contents of the plate into a sink or proper waste container. Repeat this procedure for a total of four times. After final wash, invert plate, and blot dry by hitting plate onto absorbent paper or paper towels until no moisture appears. Note: Hold the sides of the plate frame firmly when washing the plate to assure that all strips remain securely in frame. Automated Washing - Aspirate all wells, then wash plates four times using Wash Buffer (1X). Always adjust your washer to aspirate as much liquid as possible and set fill volume at 350L/well/wash. After final wash, invert plate, and blot dry by hitting plate onto absorbent paper or paper towels until no moisture appears. 5. Add Substrate A 50l and Substrate B 50l to each well. Gently mix and incubate for 15 minutes at 37C. Protect from light. 6. Add 50l Stop Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or the color change does not appear uniform, gently tap the plate to ensure thorough mixing. 7. Read the Optical Density (O.D.) at 450 nm using a microtiter plate reader within 15 minutes. CALCULATION OF RESULTS This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (X) axis versus the corresponding concentration on the horizontal (Y) axis. First, calculate the mean O.D. value for each standard and sample. All O.D. Values are subtracted by the mean value of the balnk well before result interpretation. Construct the standard curve using graph paper or statistical software. To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration. Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve. Intra-assay CV(%) is less than 10% and Inter-assay CV(%) is less than 15%. Assay range: 3.75 pg/mL  120 pg/mL. 7. Sensitivity: The minimum detectable dose of Rabbit IL-6 is typically less than 0.1 pg/mL. 8. Cross-reactivity: This assay recognizes recombinant and natural Rabbit IL-6. No significant cross-reactivity or interference was observed. 9. Storage: 2-8! (Use frequently); six months (-20!)0 10. Standard curve  FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING! TQ}v~ހN }6IL-6 ՋBRvELISA O(uffN ,gՋBRv(uNSOY[ϑhKm@n0@Fm0~~SFmSvsQmSO7h,g-NTQ}v~ހN }6IL-6 v+Tϑ0 gHeg6*Ng OX[agN2-8! [St ՋBRvǑ(uSbSONek9Y_lvTMQu8TDՋELISA 0_HQSTQ}v~ހN }6IL-6 UcbSOvS_T[-N O!kReQh,g0hQT0HRPhvhKmbSO ~Ǐ)nv^{_^mm0(u^irTMB>fr TMB(WǏ'lSirvvPS NlSb݄r v^(Wxv\O(u NlSbg~vĞr0rvmEmT7hT-NvTQ}v~ހN }6IL-6 HTckvsQ0(uvhN(W450nm l NKm[8TIQ^OD

en2\eb4!ǏY 6qT1000gy_20 R S NnsSS b\ NnnN-20!b-80!OX[ FO^MQS YQ0 2. @Fm I4I4#@#@2^2\`\ 2.H^H\`\ H)^\`\ .^\`\ .4^4\`\ 4) ^ \`\  .| ^| \`\ | . ^ \`\  )^\`\ ..H^H\`\ H)^\`\ .^\`\ .4^4\`\ 4) ^ \`\  .| ^| \`\ | . ^ \`\  )^\`\ .^\`\ .H^H\`\ H)^\`\ .^\`\ .4^4\`\ 4) ^ \`\  .| ^| \`\ | . ^ \`\  )^\`\ . ^\`\ OJQJo(l H^H\`\ HOJQJo(n ^\`\ OJQJo(u ^\`\ OJQJo(l 4^4\`\ 4OJQJo(n  ^ \`\  OJQJo(u | ^| \`\ | OJQJo(l  ^ \`\  OJQJo(n ^\`\ OJQJo(u.^\`\.H^H\`\)^\`\.^\`\.4^4\`\) ^ \`\.| ^| \`\. ^ \`\)^\`\.^\`\.H^H\`\)^\`\.^\`\.4^4\`\) ^ \`\.| ^| \`\. ^ \`\)^\`\.#@I4 &66666666(uEDTAb }\O:NbQBRǑƖh,g v^\h,g(WǑƖTv30RQN2-8! 1000gy_15R S NnsSShKm b\ NnnN-20!b-80!OX[ FO^MQS YQ0 3. ~~SFm(uQvPBS (0.01M, pH=7.4)Qm~~ SdkYu@mSFm-Nˆ㉄v~~ހOq_TKmϑ~g y͑T\~~jRx0\jRxv~~N[^SOyvPBSN, c1:9v͑ϑSOyk kY1gv~~7hT[^9mLvPBS wQSOSOyS9hnc[S_te v^ZP}YU_0cP(WPBS-NReQˆ}vvb6RBR ReQstSFmhV-N NQ NEQRxx0:NNۏNekˆ~~~ހ SN[SFmmۏLX4xx bS YQ0gT\SFmmN5000gy_5~10R S NnhKm0 4. ~ހW{Qir NnbvQ[uirh,g1000gy_20R S NnsSShKm b\ NnnN-20!b-80!OX[ FO^MQS YQ0 lh,gn@Oq_TgThKm~g Vdkn@h,g N[ۏLdkyhKm0 *gcOvՋBRThVPg vhN450nm ؚ|^R7hhVSg4Y0.5-10uL02-20uL020-200uL0200-1000uL 37!R`)n{ 4lbSyP[4l ՋBRv~b Ty96T[Mn48T[MnYl_T[vhg8T[12ag8T[6agehQT0.3mL*6{0.3mL*6{e7h,gzʑm6mL3mLehKmbSO-HRP10mL5mLe20mmQm25mL15mL cffNۏLzʑ^irA6mL3mLe^irB6mL3mLe~bkm6mL3mLe\g2 _2 _effN1N1Ne\1*N1*NeYl 1. hQTSm^O!k:N12006003001507.503.75 pg/mL 2. ~Ǐ'Yϑck8^h,gh h,gvck8^Sm^frm^HTerb_Emvr ]~S݄v^irm NO(u0 MQՋBRTh,gvNSalgNMQ b~g0 (WPX[T)neMQ:_IQvcgq\0 s^a[)nTQSb_[\N24lnQZ(WQgag N0 NUOS^ՋBR Nco}vnBRbo}vnBR@bceSv:_plSO0NUOo}vbRO4xOWՋBRv-NS^ՋBRvuir;m'`0 NO(uǏgNT0 YgS Oduu @b gv7hT^{t}Y cgqĉ[v z^Yt7hTThKmňn0 ՋBRQY ՋBRvNQυsX-NSQ^(W[)ns^aTeSO(u0 20mmQmvzʑ4l c120zʑ sS1N20mmQmR19N4l0 d\Oek N[)ns^a20minTvݔ{-NSQ@bgag iRYOgag(u\[\>eV4!0 nhQTT[T7h,gT[ hQTT[TR N TSm^vhQT50L 7h,gT[-NReQ_Km7h,g50Lzz}vT[ NR0 dzz}vT[Y hQTT[T7h,gT[-NkT[ReQ9hǏ'lSirvHRP hvhKmbSO100L (u\g\OOS^T[ 37!4ltmbR`)n{)n60min0 _SmSO 8T4l~ Nbr^ kT[Rnmmm350L Yn1min )uSmmm 8T4l~ Nbr^ Ydk͑ Ymg5!k_NS(umg:gmg 0 kT[ReQ^irA0BT50L 37!IQu[15min0 kT[ReQ~bkm50L 15minQ (W450nmlYKm[TT[vODBDJL·zobWJ?2CJPJ aJ@EHKHCJPJ aJEHKHCJPJ aJ@EHKHCJPJ aJEHKHCJPJ aJ@EHKHCJPJ aJEHKHCJPJ aJ@EHKHCJPJ aJEHKHCJPJ aJ@EHKHCJPJ aJEHKHCJPJ aJ@EHKHCJPJ aJEHKHCJPJ aJ@EHKHCJPJ aJEHKHCJPJ aJ@EHKHCJPJ aJEHKHCJPJ aJ@EHKHLNPRZ~@BD  " ( D H ·yvspjb_\TQCJCJPJ KH\CJCJCJPJ KH\ CJKH\CJCJCJCJPJ o(6KHCJPJ 6KHCJPJ 6KH CJ5KH CJ5KHCJPJ aJ5@KH\CJCJo(CJCJPJ aJ@KHCJo(aJ@KHCJCJCJo(CJCJo(CJCJo(CJCJPJ aJ@EHKHCJPJ aJEHKHH J \ ^ h j l n r ~ !!!!!!>!@!L!N!X!\!^!v!x!ÿ|yvsokCJo(CJo(CJCJCJCJCJCJo( CJo(KHCJCJCJCJCJCJo(CJCJCJo(CJCJCJCJo(CJCJCJCJo(CJCJCJo(CJCJo(CJCJCJo(CJCJo(CJo( CJo(KHCJKH CJo(KHCJKH)x!!!!!!!!!!!!!!!!!!! """"$"("T"V"X"Z"\"`"v"x"z"|"~""""""ÿ}xupkCJaJCJaJCJCJKHCJaJCJaJCJaJCJaJCJCJCJaJCJaJCJaJCJaJCJCJKHCJCJCJCJCJ5CJo(CJCJCJCJCJCJCJo(CJCJCJCJCJCJCJo(CJCJCJCJ(""""""""## ##@#J#L#N#V#####T$V$X$Z$p$r$%%&&^'`'ÿ{rkf_ZCJKH CJPJ KHCJKH CJPJ KHCJKHmHsH CJPJ KHCJ5KHmHsHCJPJ aJ5@KH\CJo(CJo(CJo(CJCJo(CJCJo(CJCJo(CJo(CJo(CJQJ CJQJo(CJQJ CJQJo(CJo(CJCJo(CJCJo( CJo(5CJ5CJaJCJaJ `'''))))**++@-B---D/F/////0 000R1T1V1{sle^YUCJo(CJKH CJPJ KH CJPJ KH CJPJ KHCJPJ o(KH CJPJ KHCJKH CJPJ KH CJPJKH CJPJ KHCJKH CJPJ KHCJKH CJPJ KHCJKH CJPJ KHCJPJ o(KH CJPJ KHCJKH CJPJ KHCJKH CJPJ KHCJKH CJPJ KHCJKH CJPJ KHV11111111122222z2222222224 4qkb[PFCJo(aJ@KHCJPJ aJ@KH CJaJKHCJPJ aJKH CJ5KHCJPJ aJ5@KH\CJPJCJPJCJPJ CJPJo(CJPJ CJPJo(CJPJ CJPJo(CJPJ CJPJo(CJPJ CJPJ5CJPJo(5 CJPJ5 CJ5KHCJPJ aJ5@KH\CJPJ o(aJ5@KH\CJPJ aJ5@KH\ 4"4$4*4,44464:4<44444444444444444ļwnc[RK@CJPJ aJ@KH CJaJKHCJPJ aJKHCJo(aJKHCJPJ aJ@KHCJPJ aJKHCJPJ aJ@KHCJo(aJ@KH CJaJKH CJo(>*CJ>* CJo(>*CJ>* CJo(>*CJPJ o(KH CJPJ KHCJPJ o(KH CJPJ KHCJPJ o(KH CJPJ KHCJo(aJKH CJaJKHCJPJ aJ@KHCJPJ aJ@KH445(5B5L5b5f55555555555555555555¿xmbYRJCJo(aJKH CJaJKHCJPJ aJKHCJPJ aJ@KHCJPJ aJ@KHCJPJ aJKHCJPJ aJ@KHCJPJ aJ@KHCJPJ aJKHCJPJ aJ@KHCJPJ aJ@KHCJPJ aJKHCJCJPJCJo(CJPJCJCJo( CJo(>*CJo(CJCJo(CJCJo( CJaJKHCJPJ aJKH56 6 666666666 6d6f666677888Ǽ{phaYRJC CJPJ KHCJPJ o(KH CJPJ KHCJPJ o(KH CJPJ KHCJPJ o(KHCJPJ o(5KH\CJPJ 5>*KH\ CJPJ KH CJPJ KHCJo(aJKH CJaJKHCJo(aJKH CJaJKHCJo(aJ@KHCJPJ aJ@KHCJPJ aJKHCJPJ aJ@KHCJPJ aJKHCJPJ aJ@KHCJPJ aJKH CJaJKH888:::::::B;J;n;z;T=V=X=\=^=f=x========">&>*>.>}zvsolgd_YPMCJCJPJ aJKH CJo(KHCJKHCJCJKHCJCJ>*CJCJo(CJCJo(CJCJo(aJKH CJaJKHCJo(aJKH CJPJ KH CJPJ KHCJPJ o(KH CJPJ KHCJPJ o(KH CJPJ KHCJPJ o(KHCJPJ 5>*KH\ CJPJ KH CJPJ KHCJPJ o(KH CJPJ KHCJPJ o(KH.>R>T>V>X>\>^>j>l>p>>> ? ?????@@@@"@$@&@,@.@žwj_RCJPJ aJ@EHKHCJPJ aJEHKHCJPJ aJ@EHKHCJo(aJKH CJaJKHCJo(aJKHCJo(CJCJPJCJCJPJCJo(CJPJCJCJPJ CJPJ>*CJPJ CJaJKHCJPJ aJKHCJPJCJo(aJKH CJaJKHCJo(aJKH CJaJKH CJ5>*CJPJ5>*.@0@4@6@8@:@>@D@F@H@L@R@T@V@X@Z@\@^@µxm`SF;.CJPJ aJ@EHKHCJPJ aJEHKHCJPJ aJ@EHKHCJPJ aJ@EHKHCJPJ aJ@EHKHCJPJ aJEHKHCJPJ aJ@EHKHCJPJ aJEHKHCJPJ aJ@EHKHCJPJ aJ@EHKHCJPJ aJEHKHCJPJ aJ@EHKHCJPJ aJEHKHCJPJ aJ@EHKHCJPJ aJ@EHKHCJPJ aJEHKHCJPJ aJ@EHKH^@b@d@h@j@p@r@v@x@z@~@@@@@@@@ķ|odWJ?2CJPJ aJ@EHKHCJPJ aJEHKHCJPJ aJ@EHKHCJPJ aJ@EHKHCJPJ aJEHKHCJPJ aJ@EHKHCJPJ aJEHKHCJPJ aJ@EHKHCJPJ aJEHKHCJPJ aJ@EHKHCJPJ aJEHKHCJPJ aJ@EHKHCJPJ aJEHKHCJPJ aJ@EHKHCJPJ aJEHKHCJPJ aJ@EHKHCJPJ aJEHKH@@@@@@@@@@@@@@@@@@ķxm`SH;0CJPJ aJEHKHCJPJ aJ@EHKHCJPJ aJEHKHCJPJ aJ@EHKHCJPJ aJ@EHKHCJPJ aJEHKHCJPJ aJ@EHKHCJPJ aJ@EHKHCJPJ aJEHKHCJPJ aJ@EHKHCJPJ aJ@EHKHCJPJ aJ@EHKHCJPJ aJEHKHCJPJ aJ@EHKHCJPJ aJEHKHCJPJ aJ@EHKHCJPJ aJEHKH@@@@@@@@@@@@@ AABB>C@CPCzoaWPHA92 CJPJ KHCJPJ o(KH CJPJ KHCJPJ o(KH CJPJ KHCJ5KHmHsHCJPJ aJ5@KH\CJPJ aJEHKHCJPJ aJEHKHCJPJ aJEHKHCJPJ aJ@EHKHCJPJ aJEHKHCJPJ aJ@EHKHCJPJ aJEHKHCJPJ aJ@EHKHCJPJ aJ@EHKHCJPJ aJ@EHKHCJaJ@EHKHCJPJ aJ@EHKHPCRCCCHD\D*E,EGGHHHHHII2IT@TBTFTJTLTNTPTTTVTXTZT\T`TfThTlTnTxTzTſ~ytoje`ZUPCJaJCJaJ CJo(aJCJaJCJaJCJaJCJaJCJaJCJaJCJaJ CJo(aJCJaJ CJo(aJCJaJCJaJ CJo(aJCJaJ CJo(aJCJaJCJaJ CJaJ5 CJaJ5 CJaJ5 CJaJ5 CJaJ5 CJaJ5 CJaJ5 CJaJ5CJo(5KHCJo(5fH q zT~TTTTTTTTTTTTTTTTTTTTTTTTTTUUUUU U&U½|wrmhc^YCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJ CJo(aJCJaJ!&U(U.U0U2U6UUDUFULUNUPUTUZU\UbUdUjUlUnUrUxUzU~UUUUUUUUUUþ}xsnid_ZCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJ!UUUUUUUUUUUUUUUUUUUUVVVxV|VVVVVV^Wú|vql^PMCJCJo(5fH q CJo(5fH q CJaJCJaJ CJo(aJCJaJ CJo(aJ CJo(aJCJQJ CJQJo(CJQJCJaJ CJo(aJCJaJCJaJCJaJ5\CJo(aJ5\CJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJCJaJ^W`WWWWW(X*XTXVXvXxXXXYY&Y(YpYrYtYvY~YYYYYZZZZZbZdZZZZ{wrmhc^CJaJCJaJCJaJCJaJCJaJCJo(CJo(5fH q CJaJCJaJCJaJCJo(CJCJCJo(5fH q CJo(5fH q CJCJCJCJ@CJCJCJCJCJCJCJCJCJCJCJCJCJCJCJCJCJ$ZZZZZZZZZZj[l[[[[[[[2\4\\\\\\\\\\\\\\Ŀ}olhea^ZWSCJo(CJCJo(CJCJo(CJCJo(CJCJo(5fH q CJo(5fH q CJaJCJaJCJaJCJaJCJaJCJaJCJaJ CJo(aJCJaJ CJo(aJCJaJCJaJCJaJ CJo(aJCJaJCJaJCJaJ CJo(aJCJaJ CJo(aJCJaJ CJo(aJ \]]$]&]*],].]0]:]<]>]F]H]^]`]t]v]x]]]]]]]]]]]]] ^{vqlfa[VCJaJ CJo(aJCJaJ CJo(aJCJaJCJaJCJaJCJaJCJaJ CJo(aJCJaJ CJo(aJCJaJCJaJCJaJ CJo(aJCJaJ CJo(aJCJaJ CJo(aJCJaJCJo(5fH q CJo(5fH q CJaJCJCJo(CJCJo(CJCJo(CJ ^^^^^^^x^|^~^^^^^^^^^^^^^^ CJo(aJCJaJ CJo(aJ CJo(aJ o(aJU0JmHsHnHtHaJ Uo(aJ 0Jo(aJ o(aJU0JU0JUPJo(mH sH nHtH jU CJo(aJCJaJ CJo(aJCJaJ CJo(aJ02^ ` b d f h j l n za$$8$7$a$$8$7$a$$8$7$a$$8$7$a$$8$7$a$$8$7$a$$8$7$ a$$8$7$` da$$8$7$H$ da$$8$7$H$ dHa$$8$7$H$ dHa$$8$7$H$ dHa$$8$7$H$8$7$n   0 lnNqdh^WDR]`] hdh^WDR]`] h dh8$7$H$ dh8$7$H$dh8$7$H$WD` dh8$7$H$ a$$8$7$H$a$$ dHa$$8$7$H$a$$8$7$ dHa$$8$7$H$a$$8$7$a$$8$7$ NPRRD dha$$$If dha$$8$7$H$dhdhdhdhdh dh8$7$H$ dh8$7$H$dhdh^WDR]`] hdh^WDR]`] h" F H j F:. dha$$$If dha$$$If$$If:V 44l44l0`F\     5 5K dha$$$If dha$$$If RF:. dha$$$If dha$$$If dha$$$If$$If:V 44l44l0+F\     5 5K dha$$$If !!!^RF: dha$$$If dha$$$If dha$$$If$$If:V 44l44l0F\     5 5K!!@!N!Z!^RF: dha$$$If dha$$$If dha$$$If$$If:V 44l44l0F\     5 5KZ!\!x!!!^RF: dha$$$If dha$$$If dha$$$If$$If:V 44l44l0`F\     5 5K!!!!!^RF: dha$$$If dha$$$If dha$$$If$$If:V 44l44l0qF\     5 5K!!!!!^RF: dha$$$If dha$$$If dha$$$If$$If:V 44l44l0`F\     5 5K!!""&"^RF: dha$$$If dha$$$If dha$$$If$$If:V 44l44l0qF\     5 5K&"("V"Z"^"^RF: dha$$$If dha$$$If dha$$$If$$If:V 44l44l0`F\     5 5K^"`"x"|""^RF: dha$$$If dha$$$If dha$$$If$$If:V 44l44l0`F\     5 5K"""""^RF: dha$$$If dha$$$If dha$$$If$$If:V 44l44l0`F\     5 5K"""N#X$^M>*dha$$VDd^WD`dhWDd` hdhh^h` h$$If:V 44l44l0`F\     5 5KX$Z$r$%&`'')sU & F 2dh8$7$H$^` 2 & F 2dh8$7$H$^` 2 & F 2dh8$7$H$^` 2 & F 2dh8$7$H$^` 2 & F 2dh8$7$H$^` 2 dha$$8$7$H$dha$$))*B--F//iK & F 2dh8$7$H$^` 2 & F 2dh8$7$H$^` 2 & F 2dh8$7$H$^` 2 & F 2dh8$7$H$^` 2 & F 2dh8$7$H$^` 2 & F 2dh8$7$H$^` 2/0T1V1122$446f6:V=wrdh dh8$7$H$dhdhdhdh8$7$ dha$$8$7$H$dh^WDR]`] dha$$8$7$H$dh & F 2dh8$7$H$^` 2 & F 2dh8$7$H$^` 2 V=V>@@@ARC,EGHIi & F dh^`  & F dh8$7$H$^`  & Fdh8$7$H$^` & Fdh8$7$H$^` & Fdh8$7$H$^` dha$$8$7$H$dhdhdh8$7$dh IbIJUFUNUA;2) a$$$If a$$$If$If$$If:V TT44l44l0\ -5* 5o55 NURUTU\UdU82) a$$$If$If$$If:V TT44l44l0\ -5* 5o55 a$$$IfdUlUpUrUzU/)$If$$If:V TT44l44l0\ -5* 5o55 a$$$If a$$$IfzUUUUU&$$If:V TT44l44l0\ -5* 5o55 a$$$If a$$$If a$$$IfUUUUU a$$$If a$$$If a$$$If$If 0@P`p6666 0@P`p 0@P`p 0@P`p 0@P`p 0@P`p 0@P`pF@Fcke a$$1$CJKHmH sH nHtH_H$A@$؞k=W[SONi@Nnfh.@^@@@PCIKM~M NP TzT&UU^WZ\ ^^0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTn N !Z!!!!&"^"""X$)/V=IMJO T@T`TTTTTU4UNUdUzUUUUVY0]^^UVWXYZ[\]^_`abcdefghijklmnopqrstuvwxyz{|}g+Gz Times New Roman-([SO;Wingdings7$*{$  Calibri?4 .Cx @Courier NewY$Myriad Pro Light Cond[SOG$   Myriad Pro Cond3$ .[x @ArialQ$KACMFI+Arial,Bold[SOG$ KACMCF+Arial[SOU$KACMGJ+Arial,Italic[SOm TimesNewRomanPS-BoldItalicMT-IdўSOO$ |i0GulimMalgun GothicG$ MFKKCL+Arial[SOU$MFKKFN+Arial,Italic[SOQ$IBFNAJ+Arial,Bold[SOG$ IBFMNH+Arial[SOG$  * HelveticaArial]$Helvetica-BoldSegoe Print Normal.wptFOR RESEARCH USE ONLY. 0FE076201A4D470 Nwmyuir QhGp| Ll$ NqZ&!),.:;?]}    & 6"0000 0 0 00000 =@\]^([{  0 0 00000;[nn)?'*2j# I | > ]mEf;PyoLhx " )$%%&W&T(((*)"+g*-'.0p37&G7e7r7UUUUUA;2) a$$$If a$$$If$If$$If:V TT44l44l0\ -5* 5o55 UUUUV82,8$7$H$8$7$H$$$If:V TT44l44l0\ -5* 5o55 a$$$IfVVVV`WWW*XVXxXXY(YtYvYY a$$8$7$H$d & Fdh & Fdh & Fdh & Fdh & Fdh & Fdh & Fdh & Fdh & Fdh & Fdh8$7$H$8$7$H$8$7$H$YYZZZdZZZl[[4\\\\.]0]8$7$H$dh8$7$H$WD`dh & Fdh & Fdh & Fdh & Fdh & Fdh & Fdh & Fdh8$7$H$ dh8$7$H$ dh8$7$H$0]<]x]]]^z^|^^^^^^}m 9r h]h 9r  9r (d2R2 9r &`#$ 9r h]h 9r  9r 9r &`#$ 9r a$$&dP 9r  & Fdh & Fdh & Fdh & Fdhdh  ^^^^ & Fdh30P. A!n#n"n$n%S2P18Fȁd:N.Πl/8JFIF+,C    $.' ",#(7),01444'9=82<.342C  2!!22222222222222222222222222222222222222222222222222" }!1AQa"q2#BR$3br %&'()*456789:CDEFGHIJSTUVWXYZcdefghijstuvwxyz w!1AQaq"2B #3Rbr $4%&'()*56789:CDEFGHIJSTUVWXYZcdefghijstuvwxyz ?( ( ( (o¾[pʙFZ=#zpe'~&x.ndgl#>j~0E绺jF*(+?? OP8l@xWk6LJŢbk鿇4 MZ%-lC*HMdk ]1fOR"|3a0A)O žm~t'ߺ۠((((((|'~$:MӴ+I?{G<%*&5by 񏊭tw3O'5z_}Ku(sd } U4:H-8vG`+znȣ$|{:o1[q pjBS,nAUz ׺G#:[on]ּ[?.fBt2&=5"ƊUFg3Fp"H9cԜz?h_2TЊQ:&hFRC_wis֑ep~X#vfDӢ4HO8Q%| '?,ݨi9?_^x7Ŷcg>]y_ʾeWR20A_"]Qd{x_,tSK@[[\ykP:0Ak>xCL ˛Qر{]QEQEQEQEgkͯKRos+Yo5 Iqw.8T~|oA}qWu«!?@_e\ď?kyώ;~=+>ѵK x CEwg7·5_6z0+^ĖQb 8Y=Ogjqmvw<,((+|+I3ۧ=+$I8|^,{;Y7iy1EҀ8 -GŞ$=_Oy'k ~-fwjO>5*aS^@Q@|[|S  ֕@?iѷ? OlR WՕOoZ)}HM}-@Q@^#m|K&A2v5vOijYZ+)Wr +jv("l >}Bug+m@0RuY_#cB4"·}nC)<(h(k v=o] Q`M}k?RxL$?N'.u&-ncz,әIc[?=+?7Ov O¬|cH_<6.Ŀ־n?n<]N=Zˈ?@lQUo`l`It Պ+þ?xv1V\Or<˲+ ~x$ʼnqs:m͞µr|#<%k(ia\)_˥uTQEQEVfY%ԴkvJӮ_#Ƴ.Pw>UxH^G4{F$ۻ\7-Kkim|\r7c]QEQEd`;?T׶mӵdZƹNxRK>& yXr8i^eBMcŚ].pO B1e# @=Q@Q@Q@/vlq1XE껏'||]1%$<3){JYsqʟξ@u IkiĊaq@HF*r }]ý2܅hm8|k(#ӴcLV4}?A_ h6 br&6((~-cŽ08Y@# $Ҽo^FZ+س`~D V}+[R&p}Ucƻeogyp]hcl#`c~*_4`).:o_?Z_ [ᙘ]<\Z2=rG}ս)TҦ 9Rzd*kk5;_A3}E|) A[G$o}E|) A[G$o}MxAEa|- A[G$o}ґc SOH5 ? +{h+OH5 ? +{h+OH5 ? +{h[a8`+guywƪF'hNƃٽww@% G ־V&-Re%@=Kмo\"PO3:~W ~ [xB=cV/5UTހ=^W H}(1((((((ҽ¿Ҋ(^(j:8:q;:8=?TA'AYCPFyGHLcTP\S2U1V+W[ XY,ZsZ <[FI\i]"D_<`sa-be euHfh|iJWj>Vl mZCmtm InGpxp>v~wxzO {i{}<s=2IP^r|1vx3aQ8sotb42]exOP(j I ?l,Rg::(`5G(~ P'>Fgac a#b2Mx #$*yokR.I"v @:\,8[ lf( i{@/ Qd)iw_Ra_Og+3>ZZZ]   ?b$o?:B-N1ҘR$ȁd:N.Πl/85R$,iN:+ʼd M (  |  JA(8 !VGr 8"6 S ?I g++T3:>FHg+!! OLE_LINK1 +g+<+g+@